<p class="jove_content">This protocol presents a simple and efficient method to isolate, identify and quantify immune cells residing in the myocardium of mice during steady state or inflammation. The protocol combines enzymatic and mechanical digestion for the generation of a single cell suspension that can be further analyzed by flow cytometry.</p>

isolation-identification-extravascular-immune-cells

Isolation and Identification of Extravascular Immune Cells of the Heart

The immune system is an essential component of a healthy heart. The myocardium is home to a rich population of different immune cell subsets with ...

<p class="jove_content">This article will detail the protocol for measuring calpain activity in fixed and living cells using flow cytometry.</p>

measuring-calpain-activity-in-fixed-and-living-cells-by-flow-cytometry

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

Calpains are ubiquitous intracellular, calcium-sensitive, neutral cysteine proteases 1. Calpains play crucial roles in many physiological processes, ...

Institution - University Health Network (UHN)

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