<p class="jove_content">To cultivate neural crest stem cells (NCSC) in vitro, a special medium (NCSCM) is required. Essential part of NCSCM is chick embryo extract (CEE). We here describe accurate techniques to produce a maximized amount of pure and high quality CEE, including details as the isolation, maceration, centrifugation, and filtration processes.</p>

production-chick-embryo-extract-for-cultivation-murine-neural-crest

Institution - University Children's Hospital Essen

Articles Published in JoVE