<p class="jove_content">To maximize the potential benefits of pulmonary gene therapy, widespread and uniform topical delivery of a viral vector across the surface epithelium is an important goal. Here, we demonstrate an aerosolization technique using a microsprayer positioned intratracheally to deliver a viral vector to newborn pig airways.</p>

intratracheal-aerosolization-of-viral-vectors-to-newborn-pig-airways

Intratracheal Aerosolization of Viral Vectors to Newborn Pig Airways

Gene therapy for airway diseases requires efficient delivery of nucleic acids to the intrapulmonary airways. In small animal models such as mice, gene ...

<p class="jove_content">Immunocytochemical identification of peripheral sensory nerve fiber subtypes (and detection of protein expression therein) are key to the understanding of molecular mechanisms underlying peripheral sensation. Here we describe methods for preparation of peripheral/visceral tissue samples, such as skin and limb bones, for specific immunostaining of peripheral sensory nerve fibers.</p>

tissue-preparation-immunostaining-mouse-sensory-nerve-fibers

Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones

Detection and primary processing of physical, chemical and thermal sensory stimuli by peripheral sensory nerve fibers is key to sensory perception in ...

Institution - The University of Iowa

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