conducting-reactions-below-room-temperature

Conducting Reactions Below Room Temperature

Source: Laboratory of Dr. Dana Lashley - College of William and Mary
Demonstration by: Matt Smith
When new bonds are formed in the course of a chemical ...

We present a two-part protocol that combines fluorescent calcium imaging with in situ hybridization, allowing the experimenter to correlate patterns of calcium activity with gene expression profiles on a single-cell level.

fluorescent-calcium-imaging-subsequent-situ-hybridization-for

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Spontaneous intracellular calcium activity can be observed in a variety of cell types and is proposed to play critical roles in a variety of physiological ...

The zebra finch (Taeniopygiaguttata) is a valuable model organism; however, early stages of zebra finch development have not been extensively studied. The protocol describes how to dissect early embryos for developmental and molecular applications.

dissection-downstream-analysis-zebra-finch-embryos-at-early-stages

Dissection and Downstream Analysis of Zebra Finch Embryos at Early Stages of Development

The zebra finch (Taeniopygiaguttata) has become an increasingly important model organism in many areas of research including toxicology1,2, behavior3, and ...

Xenopus laevis provides an ideal model system for studying cell fate specification and physiological function of individual retinal cells in primary cell culture. Here we present a technique for dissecting retinal tissues and generating primary cell cultures that are imaged for calcium activity and analyzed by in situ hybridization.

dissection-culture-analysis-xenopus-laevis-embryonic-retinal

Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue

The process by which  the anterior region of the neural plate gives rise to the vertebrate retina  continues to be a major focus of both clinical and ...

Institution - College of William and Mary

Articles Published in JoVE