<p class="jove_content">A technique to genetically manipulate epithelial cells within whole <em>ex vivo</em> cultured embryonic mouse submandibular glands (SMGs) using viral gene transfer is described. This method takes advantage of the innate ability of SMG epithelium and mesenchyme to spontaneously recombine after separation and infection of epithelial rudiments with adenoviral vectors.</p>

genetic-modification-recombination-salivary-gland-organ

Genetic Modification and Recombination of Salivary Gland Organ Cultures

Branching  morphogenesis occurs during the development of many organs, and the embryonic  mouse submandibular gland (SMG) is a classical model for the ...

Institution - University at Albany, SUNY

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