This protocol describes the production of recombinant human SLFN14 ribonuclease and its stoichiometric analysis by mass photometry.

rapid-production-recombinant-human-slfn14-ribonuclease-stoichiometric

Rapid Production of Recombinant Human SLFN14 Ribonuclease and Stoichiometric Analysis by Mass Photometry

Ribonucleases are a specialized class of RNA binding proteins that catalyze RNA cleavage and contribute to diverse biological processes including gene ...

Described here is a procedure for obtaining long stretches of current recording from one ion channel with the cell-attached patch-clamp technique. This method allows for observing, in real time, the pattern of open-close channel conformations that underlie the biological signal. These data inform about channel properties in undisturbed biological membranes.

one-channel-cell-attached-patch-clamp-recording

One-channel Cell-attached Patch-clamp Recording

Ion channel proteins are universal devices for fast communication across biological membranes. The temporal signature of the ionic flux they generate ...

Graduate Program in Neuroscience

SMBS

Institution - University at Buffalo, SUNY

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