A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is presented.

green-fluorescent-protein-based-expression-screening-membrane

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

The production of recombinant membrane proteins for structural and functional studies remains technically challenging due to low levels of expression and ...

Institution - MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus

Articles Published in JoVE