<p class="jove_content">We describe a modified method for membrane fraction isolation from human cells and sample preparation for the detection of SCAP <em>N</em>-glycosylation and total protein by using western blot. We further introduce a GFP-labeling method to monitor SCAP trafficking using confocal microscopy. This protocol can be used in regular biology laboratories.</p>

analysis-of-scap-n-glycosylation-and-trafficking-in-human-cells

Institution - The Ohio State University Comprehensive Cancer Center and College of Medicine

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