<p class="jove_content">Gene deletion mutants generated through homologous recombination are the gold standard for gene function studies. The OSCAR (One Step Construction of Agrobacterium-Recombination-ready-plasmids) method for rapid generation of deletion constructs is described. <em>Agrobacterium</em> mediated fungal transformation follows. Finally, a PCR based confirmation method of gene deletions in fungal transformants is presented.</p>

rapid-deletion-production-fungi-via-agrobacterium-mediated

Institution - NPRC, USDA-ARS

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