This protocol describes a survival procedure for safe and well-tolerated murine mesenteric lymphadenectomyat each of four stations along the mesenteric lymph node chain. This procedure is adaptable to allow for selective disruption of lymphatic communication with the distal colon, proximal colon, and/or small intestine.

murine-mesenteric-lymphadenectomy-for-selective-disruption-lymphatic

Murine Mesenteric Lymphadenectomy for Selective Disruption of Lymphatic Communication with Region-Specific Gut

Immunosurveillance versus antigen-tolerance is a fine balance within the gut microenvironment, during which the critical role of circulating T cell ...

Protocols described here allow for the study of the electrical properties of excitable cells in the most non-invasive physiological conditions by employing zebrafish embryos in an in vivo system together with a fluorescence resonance energy transfer (FRET)-based genetically encoded voltage indicator (GEVI) selectively expressed in the cell type of interest.

biosensing-motor-neuron-membrane-potential-in-live-zebrafish-embryos

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

The protocols described here are designed to allow researchers to study cell communication without altering the integrity of the environment in which the ...

Institution - Howard Hughes Medical Institute, Yale University School of Medicine

Articles Published in JoVE