<p class="jove_content">This protocol describes the synthesis, characterization, and injection of monomeric amyloid-&#946;42 peptides for generating amyloid toxicity in adult zebrafish to establish an Alzheimer&#39;s disease model, followed by histological analyses and detection of aggregations.</p>

modeling-amyloid-42-toxicity-neurodegeneration-adult-zebrafish

Modeling Amyloid-&#946;42 Toxicity and Neurodegeneration in Adult Zebrafish Brain

Alzheimer's disease (AD) is a debilitating neurodegenerative disease in which accumulation of toxic amyloid-&#946;42 (A&#946;42) peptides leads to ...

<p class="jove_content">In this article, we demonstrate a method for manipulation of gene expression in the ventricular cells of the adult zebrafish telencephalon using antisense morpholino oligonucleotides. We present this method as an efficient and quick protocol that can be used for functional studies in the adult vertebrate brain.</p>

micromanipulation-gene-expression-adult-zebrafish-brain-using

Institution - German Centre for Neurodegenerative Diseases (DZNE) Dresden within Helmholtz Association

Articles Published in JoVE