<p class="jove_content">Here, we present a protocol to investigate the intracellular mechanical properties of isolated embryonic zebrafish cells in three-dimensional confinement with direct force measurement by an optical trap.</p>

direct-force-measurements-subcellular-mechanics-confinement-using

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

During the development of a multicellular organism, a single fertilized cell divides and gives rise to multiple tissues with diverse functions. Tissue ...

<p class="jove_content">New tools for mechanobiology research are needed to understand how mechanical stress activates biochemical pathways and elicits biological responses. Here, we showcase a new method for selective mechanical stimulation of immobilized animals with a microfluidic trap allowing high-resolution imaging of cellular responses.</p>

using-microfluidics-device-for-mechanical-stimulation-high-resolution

Institution - The Institute of Photonic Sciences (ICFO)

Articles Published in JoVE