<p class="jove_content">The goal of this protocol is to provide a cell-based system that replicates the formation of alpha-synuclein aggregates <em>in vivo</em>. Intracellular alpha-synuclein inclusions are seeded in primary neurons by the internalization and propagation of exogenous administered native microsomes-associated alpha-synuclein aggregates isolated from diseased alpha-synuclein transgenic mice.</p>

exogenous-administration-microsomes-associated-alpha-synuclein

Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation

For years, the inability of replicating formation of insoluble alpha-synuclein (&#945;S) inclusions in cell cultures has been a great limitation in the ...

Institution - International School for Advanced Studies (SISSA/ISAS)

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