<p class="jove_content">We present a protocol for the real-time optical detection of single unlabeled proteins as they are secreted from living cells. This is based on interferometric scattering (iSCAT) microscopy, which can be applied to a variety of different biological systems and configurations.</p>

label-free-imaging-single-proteins-secreted-from-living-cells-via

Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

We demonstrate interferometric scattering (iSCAT) microscopy, a method capable of detecting single unlabeled proteins secreted from individual living ...

Institution - Max Planck Institute for the Science of Light (MPL)

Articles Published in JoVE