<p class="jove_content">The growth of radiolabeled bacterial cultures in microtiter dishes facilitates high throughput sampling that allows multiple technical and biological replicate assays of nucleotide pool abundance, including that of (p)ppGpp. The effects of growth transitions provoked by sources of physiological stress as well as recovery from stress can be monitored.</p>

using-microtiter-dish-radiolabeling-for-multiple-vivo-measurements

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of <em>Escherichia coli</em> (p)ppGpp Followed by Thin Layer Chromatography

The (p)ppGpp nucleotide functions as a global regulator in bacteria in response to a variety of physical and nutritional stress. It has a rapid onset, in ...

Institution - Eunice Kennedy Shriver NICHD, NIH

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