<p class="jove_content">This protocol enables efficient histone extraction from various clinical samples for subsequent post-translational modification analysis by LC-MS/MS. By facilitating robust detection of key histone modifications, it serves as a valuable tool for comprehensive epigenetic profiling in large clinical cohorts.</p>

extraction-histones-from-clinical-specimens-for-epigenetic-profiling

Extraction of Histones from Clinical Specimens for Epigenetic Profiling by Mass Spectrometry

Extensive experimental evidence supports the role of epigenetic mechanisms in cancer onset, progression, and recurrence. Among these, histone variants and ...

<p class="jove_content">Here, we describe the implementation and interpretation of the results of an in vitro mammosphere self-renewal quantitative assay.</p>

quantification-of-self-renewal-in-murine-mammosphere-cultures

Quantification of Self-renewal in Murine Mammosphere Cultures

The mammary gland is characterized by extensive regeneration capacity, as it goes through massive hormonal changes throughout the life cycle of a female. ...

Institution - European Institute of Oncology IRCCS

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