<p class="jove_content">This protocol demonstrates real-time recording of full dose-response relationships for agonist-induced GPCR activation from a single cell layer grown on a single microelectrode using label-free impedance measurements. The new dosing scheme significantly increases throughput without loss in time resolution.</p>

stepwise-dosing-protocol-for-increased-throughput-label-free

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays

Label-free impedance-based assays are increasingly used to non-invasively study ligand-induced GPCR activation in cell culture experiments. The approach ...

Institution - Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT

Articles Published in JoVE