<p class="jove_content">Translating ribosome affinity purification (TRAP) offers the possibility to dissect developmental programs with minimal processing of organs and tissues. The protocol yields high-quality RNA from cells targeted with a green fluorescent protein (GFP)-labeled ribosomal subunit. Downstream analysis tools, such as qRT-PCR or RNA-seq, reveal tissue and cell type-specific expression profiles.</p>

translating-ribosome-affinity-purification-trap-to-investigate

Translating Ribosome Affinity Purification (TRAP) to Investigate <em>Arabidopsis thaliana</em> Root Development at a Cell Type-Specific Scale

In this article, we give hands-on instructions to obtain translatome data from different Arabidopsis thaliana root cell types via the translating ribosome ...

Institution - Biophore - UNIL

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