<p class="jove_content">We describe here a protocol to characterize protein-protein interactions between two highly-differently expressed proteins in live <em>Pseudomonas aeruginosa</em> using FLIM-FRET measurements. The protocol includes bacteria strain constructions, bacteria immobilization, imaging and post-imaging data analysis routines.</p>

flim-fret-measurements-protein-protein-interactions-live

FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.

Protein-protein interactions (PPIs) control various key processes in cells. Fluorescence lifetime imaging microscopy (FLIM) combined with F&#246;rster ...

Institution - Université de Strasbourg, UMR 7242, ESBS

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