This protocol describes CRISPR-Cas9-mediated disruption of the nodC locus in Mesorhizobium ciceri using homology-directed repair and GFP-based screening to generate symbiosis-defective mutants for functional genomics studies in chickpea-associated rhizobia.

crispr-cas9-genome-editing-mesorhizobium-ciceri-through-nodc

CRISPR-Cas9 Genome Editing in Mesorhizobium ciceri Through nodC Disruption for Chickpea Symbiosis Studies

Mesorhizobium ciceri, a nitrogen-fixing symbiont of chickpea (Cicer arietinum), remains genetically challenging to manipulate using conventional ...

This study presents methodologies to study the pathomorphological and molecular mechanisms underlying chickpea&#8211;Rhizoctonia bataticola interaction. The blotting paper method is useful to rapidly study chickpea genotype responses, while the sick pot-based method can be used to simultaneously impose drought and R. bataticola infection and screen for tolerant genotypes.

dry-root-rot-disease-assays-in-chickpea-a-detailed-methodology

Dry Root Rot Disease Assays in Chickpea: a Detailed Methodology

Dry root rot (DRR) disease is an emerging biotic stress threat to chickpea cultivation around the world. It is caused by a soil-borne fungal pathogen, ...

Virus-induced gene silencing is an useful tool for identifying genes involved in nonhost resistance of plants. We demonstrate the use of bacterial pathogens expressing GFPuv in identifying gene silenced plants susceptible to nonhost pathogens. This approach is easy, fast and facilitates large scale screening and similar protocol can be applied to studying various other plant-microbe interactions.

vigs-mediated-forward-genetics-screening-for-identification-genes

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance


	Nonhost disease resistance of plants against bacterial pathogens is controlled by complex defense pathways. Understanding this mechanism is important ...

Institution - National Institute of Plant Genome Research

Articles Published in JoVE