<p class="jove_content">This article reports a simple method to isolate the outer and inner perivitelline sublayers of chicken eggs while minimizing structural alteration and to optimize protein solubilization of each sublayer for proteomic analyses.</p>

mechanical-separation-protein-solubilization-outer-inner

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

The perivitelline layer that surrounds the egg yolk plays a fundamental role in fertilization, in egg defense, and in the development of the avian embryo. ...

Institution - Université de Tours, Biologie des Oiseaux et Aviculture, 37380, Nouzilly, France

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