In a C. elegans excitotoxicity model, this protocol employs in vivo imaging to analyze the regulation of necrotic neurodegeneration, the effect of genes encoding candidate mediators, and involvement of mitochondria. Cell dissociation and sorting is used to specifically obtain at-risk neurons for cell-specific transcriptomic analysis of neurodegeneration and neuroprotection mechanisms.

live-animal-imaging-cell-sorting-methods-for-investigating

Live Animal Imaging and Cell Sorting Methods for Investigating Neurodegeneration in a C. elegans Excitotoxic Necrosis Model

Excitotoxic necrosis is a leading form of neurodegeneration. This process of regulated necrosis is triggered by the synaptic accumulation of the ...

Cellular

Molecular

and Biomedical Science

CUNY School of Medicine

PhD Program in Biology

The CUNY Graduate Center

Cellular, Molecular, and Biomedical Science, CUNY School of Medicine

PhD Program in Biology, The CUNY Graduate Center

The City University of New York (CUNY)

Institution - The City University of New York (CUNY)

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