<p class="jove_content">We present a method for the acquisition of fluorescence reporter time courses from single cells using micropatterned arrays. The protocol describes the preparation of single-cell arrays, the setup and operation of live-cell scanning time-lapse microscopy and an open-source image analysis tool for automated preselection, visual control and tracking of cell-integrated fluorescence time courses per adhesion site.</p>

live-cell-imaging-single-cell-arrays-lisca-versatile-technique-to

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

Live-cell Imaging of Single-Cell Arrays (LISCA) is a versatile method to collect time courses of fluorescence signals from individual cells in high ...

Institution - Ludwig-Maximilians-Universität München, Geschwister-Scholl-Platz 1, 80539 München, Germany

Articles Published in JoVE