<p class="jove_content">This protocol describes the collection of human aortic valves extracted during surgical aortic valve replacement procedures or from cadaveric tissue, and the subsequent isolation, expansion, and characterization of patient specific primary valve endothelial and interstitial cells. Included are important details regarding the processes needed to ensure cell viability and phenotype specificity. </p>

isolation-of-human-primary-valve-cells-for-in-vitro-disease-modeling

Isolation of Human Primary Valve Cells for In vitro Disease Modeling

Calcific aortic valve disease (CAVD) is present in nearly a third of the elderly population. Thickening, stiffening, and calcification of the aortic valve ...

Institution - University of Pittsburgh and Heart and Vascular Institute, University of Pittsburgh Medical Center

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