This protocol describes the process for obtaining human macrophages from monocytes for infection with Leishmania braziliensis. It also allows researchers to evaluate infection rate and parasite viability, ROS production by fluorescence microscopy, and the production of inflammatory mediators in culture supernatants to investigate macrophage response to infection.

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Obtainment of Macrophages from Human Monocytes to Assess Leishmania braziliensis Infection Rate and Innate Host Immune Response

Macrophages are multifunctional cells essential to the immune system function, and the primary host cell in Leishmania braziliensis (Lb) infection. These ...

Senai Institute for Innovation in Advanced Health Systems, National Service for Industrial Learning, Integrated Manufacturing and Technology Campus, SENAI - CIMATEC Salvador

Institution - Senai Institute for Innovation in Advanced Health Systems, National Service for Industrial Learning, Integrated Manufacturing and Technology Campus, SENAI - CIMATEC Salvador

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