cell-free-expression-systems-methods-of-production-and-use

Cell-Free Expression Systems: Methods of Production and Use

ARTICLES DISCUSSED:
Grasemann, L., Lavickova, B., Elizondo-Cant&#250;, M. C., Maerkl, S. J. OnePot PURE cell-free system. Journal of Visualized ...

<p class="jove_content">Well-characterized genetic parts are necessary for the design of novel genetic circuits. Here we describe a cost-effective, high-throughput method for rapidly characterizing genetic parts. Our method reduces cost and time by combining cell-free lysates, linear DNA to avoid cloning, and acoustic liquid handling to increase throughput and reduce reaction volumes.</p>

rapid-characterization-of-genetic-parts-with-cell-free-systems

Institution - US Army Combat Capabilities Development Command Chemical Biological Center

Articles Published in JoVE