Here is a protocol to identify genetic interactions through an increased copy number suppressor screen in Saccharomyces cerevisiae. This method allows researchers to identify, clone, and test suppressors in short-lived yeast mutants. We test the effect of the copy number increase of SIR2 on lifespan in an autophagy null mutant.

a-suppressor-screen-for-characterization-genetic-links-regulating

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Aging is the time dependent deterioration of an organism&#8217;s normal biological processes that increases the probability of death. Many genetic factors ...

A novel method for metal core nanoparticle synthesis using a water stable silanol is described.

generation-zerovalent-metal-core-nanoparticles-using-n-2-aminoethyl-3

Institution - William Paterson University

Articles Published in JoVE