This protocol presents an optimized method for isolating the epithelial lining of the adult female mouse urethra for downstream analyses, including flow cytometry, immunostaining, and organoid culture. Methods yielded enriched, viable urethral epithelial cells, as evidenced by flow cytometry analysis, whole-mount imaging, and efficient organoid formation.

an-optimized-method-to-isolate-female-mouse-urethral-epithelium-for

An Optimized Method To Isolate Female Mouse Urethral Epithelium For Single-Cell And Immunofluorescence Analysis

The urethra, lined by epithelial cells, serves as the conduit for urine outflow from the body. The epithelial lining of the urethra comprises distinct ...

This protocol details the enrichment of native mycobacterial extracellular vesicles (mEVs) from axenic cultures of Mycobacterium smegmatis (Msm) and how mCherry (a red fluorescent reporter)-containing recombinant MsmEVs can be designed and enriched. Lastly, it verifies the novel approach with the enrichment of MsmEVs containing the EsxA protein of Mycobacterium tuberculosis.

enrichment-native-recombinant-extracellular-vesicles

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Most bacteria, including mycobacteria, generate extracellular vesicles (EVs). Since bacterial EVs (bEVs) contain a subset of cellular components, ...

We describe a protocol for the isolation of mesenchymal stem cells from human umbilical cord tissue and their differentiation into the skeletal muscle lineage.

generation-mesenchymal-stem-cells-from-human-umbilical-cord-tissue

Institution - NCR Biotech Science Cluster

Articles Published in JoVE