<p class="jove_content">The ability to express viral vectors in the prepubertal brain is fundamental to studying the cellular and circuit mechanisms for brain development. Here, we describe a protocol for performing stereotaxic viral surgery in the infant mouse at postnatal days 11 or 12 (P11-P12) and provide guidelines for postoperative care.</p>

stereotaxic-surgery-in-infant-mice-and-postoperative-care

Stereotaxic Surgery in Infant Mice and Postoperative Care

Understanding the developmental, cellular, and circuit processes across the postnatal period is crucial for elucidating the complexities of brain ...

<p class="jove_content">Here, we present a protocol to utilize the latest version of the US Environmental Protection Agency Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) tool. This protocol demonstrates the application of the online tool to rapidly analyze protein conservation and provide customizable and easily interpretable predictions of chemical susceptibility across species.</p>

demonstration-sequence-alignment-to-predict-across-species

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation

The US Environmental Protection Agency Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) tool is a fast, freely available, online ...

Institution - Research Triangle Park

Articles Published in JoVE