<p class="jove_content">The present protocol describes the setting up of 3D <em>'</em>on top' cultures of a non-transformed breast epithelial cell line, MCF10A, that has been modified to study Platelet Activating Factor (PAF) induced transformation. Immuno-fluorescence has been used to assess the transformation and is discussed in detail.</p>

phospholipid-mediator-induced-transformation-three-dimensional

Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures

Several models have been developed to study cancer, such as rodent models and established cell lines. Valuable insights into carcinogenesis have been ...

Institution - Enveda Therapeutics India Pvt Ltd.

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