A design is presented to enable cost-effective low-temperature battery cycling for 2032 coin cells in a low-humidity environment. Cells are cycled at 0 &#176;C inside a chamber cooled by thermoelectric modules. This simple design can be adapted for experiments that require similar thermal and humidity-controlled environments or different battery geometries.

coin-cell-battery-chamber-design-for-low-temperature-operando

Coin Cell Battery Chamber Design for Low-temperature Operando Experiments

As we become more reliant on the rechargeable batteries that power our devices, it is becoming increasingly important to understand the capacity and ...

The present protocol illustrates the use of commercially available components to generate a stable and linear thermal gradient. Such gradient can then be used to determine the upper thermal limit of planktonic organisms, particularly invertebrate larvae.

thermal-limits-determination-for-zooplankton-using-a-heat-block

Thermal Limits Determination for Zooplankton Using a Heat Block

Thermal limits and breadth have been widely used to predict species distribution. As the global temperature continues to rise, understanding how thermal ...

Freshwater planarians exhibit three gaits (gliding, peristalsis, and scrunching) that are distinguishable by quantitative behavioral analysis. We describe a method to induce scrunching using various noxious stimuli, quantification thereof, and distinction from peristalsis and gliding. Using gene knockdown, we demonstrate the specificity of scrunching as a quantitative phenotypic readout.

planarian-scrunching-as-quantitative-behavioral-readout-for-noxious

Planarian Scrunching as a Quantitative Behavioral Readout for Noxious Stimuli Sensing

Freshwater planarians normally glide smoothly through ciliary propulsion on their ventral side. Certain environmental conditions, however, can induce ...

Through a double treatment with colchicine, a plant-derived toxin that kills dividing cells, nerve-free Hydra vulgaris can be generated. These Hydra cannot feed or egest on their own. This paper describes an improved method for long-term maintenance of nerve free Hydra vulgaris in the laboratory.

generation-and-long-term-maintenance-of-nerve-free-hydra

Generation and Long-term Maintenance of Nerve-free Hydra

The interstitial cell lineage of Hydra includes multipotent stem cells, and their derivatives: gland cells, nematocytes, germ cells, and nerve cells. The ...

Institution - Swarthmore College

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