Bronchial Nasal Models
Bronchial Nasal Models - Related Videos
09:50
Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke
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Cited by 40 •
2015
The aim of this protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke (CS) at the air-liquid interface. The impact of CS on the tissues is then investigated using a cytochrome P450 activity assay, a cilia beating measurement, and a systems biology approach.
14:39
Bronchial Thermoplasty: A Novel Therapeutic Approach to Severe Asthma
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Cited by 4 •
2010
Bronchial thermoplasty is a non-drug procedure for severe persistent asthma that delivers thermal energy to the airway wall in a precisely controlled manner to reduce excessive airway smooth muscle. Reducing airway smooth muscle decreases the ability of the airways to constrict, thereby reducing the frequency of asthma attacks.
10:38
Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
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Cited by 134 •
2013
Nasal epithelial cells, obtained through superficial scrape biopsy of human volunteers, are expanded and transferred onto tissue culture inserts. Upon reaching confluency, cells are grown at air liquid interface, yielding cultures of ciliated and non-ciliated cells. Differentiated nasal epithelial cell cultures provide viable experimental models for studying the respiratory mucosa.
02:46
A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization
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Cited by 4 •
2023
Various animal models of pulmonary fibrosis have been established using bleomycin to clarify the pathogenesis of pulmonary fibrosis and find new drug targets. However, most pulmonary fibrosis models targeting lung tissue have uneven drug administration. Here, we propose a model of uniform pulmonary fibrosis induced by nasal bleomycin nebulization.
14:32
Primary Human Bronchial Epithelial Cells Grown from Explants
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Cited by 20 •
2010
Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.
FAQs
An air-liquid interface exposes the upper surface of cultured airway epithelial cells to air while maintaining controlled support below. This arrangement promotes cellular differentiation and enables functions such as mucus production and ciliary activity. As a result, the model more closely reflects airway behavior relevant to studying responses to inhaled substances, pathogens, inflammation, and therapeutic compounds.
Mucus production and ciliary function provide measurable indicators of epithelial differentiation and airway performance. Changes in these features can reveal how an inhaled substance, pathogen, or treatment affects the tissue. Including both properties therefore helps bioengineers assess airway responses in a controlled setting rather than examining exposure effects through a single biological readout.
These models allow researchers to vary the biological challenge or treatment while maintaining controlled culture conditions. They can examine interactions between airway tissues and inhaled substances, pathogens, inflammatory stimuli, or therapeutic compounds. Comparing the resulting airway responses supports investigation of respiratory disease mechanisms, toxicology, drug delivery, and treatment performance within an engineered experimental platform.
Bronchial nasal models provide a controllable human airway platform that can be used without relying solely on animal studies. Researchers can expose cultured airway cells to defined substances, pathogens, or therapies and observe tissue-level responses under laboratory conditions. This complements other approaches by supporting focused respiratory experiments while preserving relevant epithelial functions such as mucus production and ciliary activity.
A typical workflow begins by culturing airway epithelial cells under controlled conditions and, when appropriate, establishing an air-liquid interface to support differentiation. Researchers then expose the engineered airway tissue to a selected inhaled substance, pathogen, inflammatory challenge, or therapeutic compound. They evaluate resulting airway responses, including changes associated with epithelial function, to address a defined research question.
The systems support several application areas, including respiratory infection studies, inflammation research, toxicology, drug delivery, and therapeutic development. They can also contribute to personalized medicine by providing a platform for examining airway responses in a controlled experimental context. Their value comes from connecting engineered tissue behavior with questions about disease, exposure, and treatment.