Pcr Microchannel
Pcr Microchannel - Related Videos
10:55
Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip
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Cited by 5 •
2013
A microchannels-on-a-chip platform was developed by the combination of photolithographic reflowable photoresist technique, soft lithography, and microfluidics. The endothelialized microchannels platform mimics the three-dimensional (3D) geometry of in vivo microvessels, runs under controlled continuous perfusion flow, allows for high-quality and real-time imaging and can be applied for microvascular research.
10:24
Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
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Cited by 16 •
2016
Collagen is a core component of the ECM, and provides essential cues for several cellular processes ranging from migration to differentiation and proliferation. Provided here is a protocol for embedding cells within 3D collagen hydrogels, and a more advanced technique for generating randomized or aligned collagen matrices using PDMS microchannels.
08:32
Assembly and Characterization of an External Driver for the Generation of Sub-Kilohertz Oscillatory Flow in Microchannels
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2022
The protocol demonstrates a convenient method to produce harmonic oscillatory flow from 10-1000 Hz in microchannels. This is performed by interfacing a computer-controlled speaker diaphragm to the microchannel in a modular manner.
08:51
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
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Cited by 9 •
2016
Here, ribosomal spacer PCR (RS-PCR) is used together with a miniaturized electrophoresis system as a fast and high resolution method for genotyping S. aureus at moderate costs allowing a high throughput.
14:12
Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
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2023
This protocol describes how to build a continuous-flow-polymerase chain system based on a microfluidic chip and how to build a capillary electrophoresis system in the lab. It presents a simple method for the analysis of nucleic acids in the lab.
FAQs
The cycle repeatedly applies denaturation, primer annealing, and polymerase-mediated extension to amplify targeted DNA. Because these stages require controlled temperature changes, the microchannel’s small dimensions allow rapid heat transfer and precise temperature control. That combination supports repeated cycling in a compact reaction environment and can shorten processing time for genetic analysis.
Miniaturization reduces the amount of sample and reagents required for each reaction. Narrow channels and small chambers also create a compact environment in which temperature changes can be controlled precisely. These features support rapid processing while conserving materials, making the format useful for systems designed for compact genetic analysis and molecular testing.
Pumps and valves can support fluid handling within an integrated microfluidic device, while sensors help monitor system conditions. Optical or electrical readouts provide ways to detect or report reaction results. Together, these components can support automation and connect amplification with measurement, helping transform a reaction chamber into a portable molecular-testing platform.
A typical workflow places the sample and required reagents in the microfluidic system, establishes controlled temperature conditions, and repeatedly carries out denaturation, primer annealing, and polymerase-mediated extension. Integrated pumps or valves may manage fluid movement, while sensors and optical or electrical readouts support monitoring. The resulting amplified DNA can then inform genetic analysis.
Researchers may select this format when they need targeted DNA amplification while limiting sample and reagent volumes. Its compact design is relevant to pathogen detection, mutation screening, and genetic analysis. The technology is especially useful when reduced processing time, integrated measurement, or a portable testing format is important to the research or diagnostic objective.
In bioengineering, PCR microchannels provide a route for combining amplification, fluid handling, temperature control, sensing, and signal readout within one compact platform. Such integration can contribute to automated devices for molecular testing and support point-of-care applications. The resulting systems are suited to portable genetic analysis, pathogen detection, and mutation screening where compact operation is valuable.