Faims
Faims - Related Videos
09:44
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
0 Views •
2022
The feasibility of whole-genome sequencing (WGS) strategies using benchtop instruments has simplified the genome interrogation of every microbe of public health relevance in a lab setting. A methodological adaptation of the workflow for bacterial WGS is described and a bioinformatics pipeline for analysis is also presented.
07:59
Transition of Farm Pigs to Research Pigs using a Designated Checklist followed by Initiation of Clicker Training - a Refinement Initiative
0 Views •
Cited by 11 •
2021
Refinement of porcine studies is achieved by introducing a standardized checklist and positive reinforcement training using a clicker. This work supports the collection of samples and the conduct of daily chores related to the animals.
06:26
Capturing Flow-weighted Water and Suspended Particulates from Agricultural Canals During Drainage Events
0 Views •
Cited by 2 •
2017
Nutrients present in particulate form can contribute significantly to the overall loads in agricultural drainage waters. This study describes a novel method to capture flow-weighted water and suspended particulates from farm canal drainage over the entire duration of the drainage event.
04:45
Population Growth - Concepts
0 Views •
2019
Population Models An ecological population is a group of individuals of a single species living in an area at the same time. To persist, a population must either grow or maintain its size. Population ecology is the study of how population size and age distribution change over time through interactions with other species and the environment as well as with individuals of their own species. A particularly important feature that is studied by ecologists is the rate at which a population grows or...
07:03
A Modified Co-Culture System for Understanding Granulosa-Theca Cell Interactions in the Bovine Ovary
0 Views •
Cited by 1 •
2025
This article presents a co-culture model of bovine theca and granulosa cells. This method has the potential to serve as a reliable foundation for analyses focused on the study of paracrine communication and substrate transport between the somatic cells within the follicle.
FAQs
FAIMS separates ions by their differential mobility, meaning the difference in how an ion responds to the high-field and low-field portions of the applied waveform. Ions with different mobility changes behave differently while traveling through the carrier gas. Adjusting the compensation voltage counterbalances this behavior for a selected ion, allowing that species to pass selectively.
The compensation voltage selects which ion has the appropriate net mobility for transmission through the FAIMS device. Because ions respond differently to the alternating field strengths, changing this voltage changes the ion population that passes onward. Scanning or setting the compensation voltage therefore provides a way to separate ion signals before mass-spectrometric detection.
The waveform creates alternating periods of high and low electric-field strength rather than applying one constant field. This exposes ions to unequal mobility responses during each part of the cycle. Those differences generate the separation that the compensation voltage can resolve, making the field pattern central to FAIMS selectivity and its ability to reduce spectral overlap.
FAIMS adds a rapid gas-phase separation based on differential mobility, whereas mass spectrometry provides ion detection and liquid chromatography offers a separate separation dimension. Because these approaches distinguish ions using different principles, combining them can improve ion identification and reduce overlap in complex chemical samples. FAIMS therefore functions as an orthogonal complement rather than a replacement.
A typical workflow sends ions through a carrier gas while an asymmetric electric waveform and compensation voltage control which ions are transmitted. The selected ions then enter the mass spectrometer for detection. Researchers can vary the compensation voltage to examine different transmitted ion populations, producing separated signals that support interpretation of complex mixtures.
FAIMS is particularly useful when a sample contains many ions whose signals may overlap during analysis. Its separation can improve identification of pharmaceuticals, metabolites, proteins, and reaction products before mass-spectrometric detection. The added mobility-based dimension is valuable for complex mixtures because it helps distinguish chemical components that might otherwise produce crowded or ambiguous spectra.