Fluorometer Spectrofluorometer
Fluorometer Spectrofluorometer - Related Videos
07:03
Measuring Photophysiology of Attached Stage of Colacium sp. by a Cuvette-Type Fast Repetition Rate Fluorometer
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Cited by 1 •
2021
Fast repetition rate fluorometer (FRRf) is a beneficial method for measuring photosystem II photophysiology and primary productivity. Here we describe a protocol to measure PSII photophysiology of epizoic alga, Colacium sp. on substrate zooplankton using cuvette-type FRRf.
09:12
G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay
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Cited by 4 •
2016
Simple methods to detect the selective activation of G proteins by G protein-coupled receptors remain an outstanding challenge in cell signaling. Here, Fӧrster resonance energy transfer (FRET) biosensors have been developed by pairwise tethering a GPCR to G protein peptides to probe conformational changes at controlled concentrations in live cells.
07:09
Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
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Cited by 1 •
2022
The study details the methodology of FRET mapping including the selection of labeling sites, choice of dyes, acquisition, and data analysis. This methodology is effective at determining binding sites, conformational changes, and dynamic motions in protein systems and is most useful if performed in conjunction with existing 3-D structural information.
02:48
Quantifying Bacterial Infection-Induced Amyloids Using Thioflavin T Fluorescence
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2025
Source: Balczon, R., et al. Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa. J. Vis. Exp. (2018)This video demonstrates the protocol to quantify amyloids in culture supernatants from pulmonary endothelial cells infected with virulent bacteria, using thioflavin T, a fluorescent dye that binds to amyloid beta-sheet structures. An increase in fluorescence compared to baseline indicates the presence of amyloids in the culture...
01:58
Studying the Permeation of FITC and FITC-Ferritin through an In Vitro Blood-Brain Barrier Model
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2025
This video demonstrates a method to study permeation through an in vitro blood-brain barrier (BBB) model using astrocytes and endothelial cells on a membrane insert, with free FITC and FITC-loaded ferritin. FITC-ferritin bound to receptors on endothelial cells and crossed the membrane barrier to the lower chamber while free FITC showed restricted permeation and remained in the upper chamber.
FAQs
Fluorescence measurement separates two wavelength roles: the sample receives light at a selected excitation wavelength, then emits light at longer wavelengths that the detector records. Changing the excitation or emission setting reveals how the substance responds across wavelengths. This distinction allows analysts to examine both the conditions that produce fluorescence and the resulting emission pattern, supporting molecular identification.
Monochromators select and vary the wavelengths used to excite the sample or measure its emitted light. By scanning these settings, the instrument generates excitation or emission spectra rather than a single fluorescence reading. Those spectra provide a fuller description of a substance’s light-emission behavior and help distinguish compounds through their spectral characteristics.
Fluorescence can offer high sensitivity and selectivity, so a fluorometer can support detection of trace compounds while focusing measurement on characteristic light-emission behavior. This combination helps analysts quantify substances at low levels and obtain chemically informative measurements from samples in which trace amounts are important.
A selected excitation wavelength is applied to the sample, and the emitted light is measured at longer wavelengths. The resulting fluorescence reading can support concentration measurement, while a scan of excitation or emission wavelengths produces a spectrum for identification. The output may therefore be either a quantitative signal or a broader spectral profile, depending on the measurement.
Chemists would use its scanning capability when an experiment requires excitation or emission spectra, such as identifying a molecular fluorescent response or examining photophysical behavior. A single selected-wavelength measurement is more suited to concentration analysis or monitoring a changing reaction signal when spectral scanning is not the primary need.
Fluorometric methods can track reaction progress, measure concentrations, identify molecular species, and investigate interactions in solutions, materials, biological samples, or environmental samples. The same measurement principle therefore supports both targeted analysis and broader chemical characterization. It is especially useful when researchers need sensitive information about trace substances or changes in light-emission behavior.