Migration Rate
Migration Rate - Related Videos
08:24
The Transwell Migration Assay
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2023
Cells migration in response to chemical cues is crucial to development, immunity and disease states such as cancer. To quantify cell migration, a simple assay was developed in 1961 by Dr. Stephen Boyden, which is now known as the transwell migration assay or Boyden chamber assay. This set-up consists an insert which separates the wells of a multiwell plate into top and bottom compartments. Cells whose migration is to be studied are seeded into the top compartment and the chemoattractant...
08:52
An Introduction to Cell Motility and Migration
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2023
Cell motility and migration play important roles in both normal biology and in disease. On one hand, migration allows cells to generate complex tissues and organs during development, but on the other hand, the same mechanisms are used by tumor cells to move and spread in a process known as cancer metastasis. One of the primary cellular machineries that make cell movement possible is an intracellular network of myosin and actin molecules, together known as “actomyosin”, which creates a...
07:42
Non-invasive Imaging of Leukocyte Homing and Migration in vivo
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Cited by 8 •
2010
Here, we describe a non-invasive two-photon (2P) microscopy approach to study leukocyte homing in the mouse footpad. We discuss the technical aspects of our tissue imaging preparation and walk the reader through a typical experiment from initial set up to execution and data collection.
02:53
Reaction Rate
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2020
The rate of reaction is the change in the amount of a reactant or product per unit time. Reaction rates are therefore determined by measuring the time dependence of some property that can be related to reactant or product amounts. Rates of reactions that consume or produce gaseous substances, for example, are conveniently determined by measuring changes in volume or pressure. The mathematical representation of the change in the concentration of reactants and products, over time, is the rate...
04:17
Nuclear Migration in the Drosophila Oocyte
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Cited by 1 •
2021
In Drosophila, the oocyte nucleus undergoes microtubule-dependent migration during oogenesis. Here, we describe a protocol that was developed to follow the migration by performing live imaging on egg chambers ex-vivo. Our procedure maintains egg chambers alive for 12 h to acquire multi-position 3D time-lapse movies using spinning-disk confocal microscopy.
FAQs
Charge determines how strongly a chemical species responds to the applied electric field. Species with different charges experience different electrical forces, while friction from the surrounding gel or solution resists movement. Comparing their migration rates can therefore help distinguish charged compounds and assess how effectively an electrophoretic system separates components in a mixture.
Molecular size and shape affect the friction a species encounters as it moves through the medium. Even compounds with similar electrical behavior may migrate at different rates if their dimensions or configurations change the resistance to movement. This dependence makes migration measurements useful for comparing proteins, nucleic acids, ions, and other chemical species.
Field strength and medium composition are important variables, alongside the species’ charge, size, and shape. Changing the electric field changes the driving force, while changing the gel or solution alters the surrounding resistance and transport environment. Controlling these conditions is essential when comparing migration rates or optimizing separation performance.
A practical comparison requires observing how chemical species move through a selected gel or solution while an electric field is applied. Their movement is then assessed as migration rate under the chosen conditions. Keeping the medium and field conditions consistent allows differences among species to support identification, comparison, and evaluation of separation quality.
Migration rate is useful when a mixture contains species that respond differently to an electric field. Measuring and comparing movement can help characterize components, distinguish ions, proteins, nucleic acids, or other compounds, and evaluate whether separation has resolved the mixture effectively. The same information can contribute to purity assessment and analytical comparison.
Changes in the migration behavior of chemical species can provide information for reaction studies by helping compare compounds present under different conditions. In electrophoresis, migration measurements also guide adjustments to field strength or medium composition. Researchers can use these comparisons to improve separation, assess analytical performance, and select conditions suited to the compounds being examined.