Wagner Turbidimeter
Wagner Turbidimeter - Related Videos
09:13
Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
0 Views •
Cited by 5 •
2017
We describe a straightforward method for the isolation of washed platelets from human blood followed by agonist-induced platelet aggregation measurements by turbidimetry. As an example we apply this method for studying the aggregation response of human platelets to collagen after a pre-incubation with the Pannexin1 channel inhibitor Brilliant Blue FCF.
07:08
A Mouse Model for Laser-induced Choroidal Neovascularization
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Cited by 87 •
2015
Here, we present the mouse laser-induced choroidal neovascularization (CNV) protocol, an experimental model that re-creates the vascular hallmarks of neovascular age-related macular degeneration (AMD). Once mastered, it can reliably and effectively induce CNV as a model system to test various experimental measures.
05:40
In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation
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Cited by 4 •
2016
Here, we present an in vivo technique for gene transfer to Schwann cells (SCs) in the rodent sciatic nerve. This simple technique is useful for investigating signaling mechanisms involved in the development and maintenance of myelinating SCs.
05:29
Free-Hand Intracerebroventricular Injections in Mice
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Cited by 7 •
2024
Here, a simple and rapid approach for performing intracerebroventricular injections in mice using a free-hand approach (that is, without a stereotaxic device) is described.
08:24
Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
0 Views •
Cited by 2 •
2022
An optimized protocol is presented that enables the depletion of cytoplasmic microtubule organizing centers in mouse oocytes during metaphase I using a near-infrared femtosecond laser.
FAQs
During a test, particles settle at rates related to their size, so the concentration remaining in suspension changes with time. Stokes’ law provides the sedimentation relationship used to interpret that change. The instrument’s optical system tracks altered light transmission as the suspension clears, converting a time-dependent optical signal into information about the powder’s fineness.
Rather than measuring each grain separately, the method observes the combined behavior of the particle population. Different particle sizes contribute differently to the suspension’s settling pattern, allowing the measurement to represent particle-size distribution and specific surface indirectly. This population-level approach is useful for powdered materials such as Portland cement, where overall fineness is the relevant engineering characteristic.
Sample dispersion is a critical condition because the measurement depends on particles settling through the liquid as a suspension. If the cement is not carefully dispersed, the observed change in concentration may not represent the intended settling behavior. Consistent preparation therefore helps the optical signal reflect sedimentation rather than an avoidable change in how the sample was suspended.
Specific surface and fineness are inferred from settling and light-transmission behavior, not obtained by individually sizing particles. That distinction makes the Wagner Turbidimeter a bulk analytical approach: it summarizes the powder through suspension behavior instead of producing a direct measurement for every particle. The result is suited to evaluating the overall condition of a cement powder.
A cement sample is first carefully dispersed in a liquid to form a suspension. The suspension is then allowed to settle while the changing concentration of particles is monitored through its optical response. As light transmission changes over time, the sedimentation behavior is interpreted using Stokes’ law to estimate the powder’s fineness and specific surface.
The key recorded behavior is the change in light transmission as settling proceeds. Because suspended-particle concentration decreases over time, this optical change provides the measurement basis for estimating fineness and specific surface. The output should therefore be understood as an indirect, suspension-based characterization rather than a direct inventory of particle diameters.
In cement engineering, the instrument supports quality control by checking powder fineness, process evaluation, and assessment of properties influenced by fineness. Its value lies in connecting a measurable settling and optical response with a powder characteristic that affects engineering behavior, while avoiding the need to size particles individually.