Plant Pathogen Detection

Plant pathogen detection is the identification of disease-causing organisms, including fungi, bacteria, viruses, and nematodes, in plants or environmental samples. It combines symptom assessment with methods such as microscopy, culture, immunoassays, and nucleic acid amplification, which detect characteristic structures, antigens, or genetic sequences from pathogens. Accurate detection supports early disease diagnosis, surveillance, quarantine, and targeted management in agricultural and natural ecosystems. It also helps researchers track pathogen spread, distinguish closely related species, and evaluate environmental conditions that influence infection, providing essential evidence for protecting plant health, biodiversity, and food production.

Plant Pathogen Detection - Related Videos

Research

JoVE Journal - Immunology and Infection
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Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants

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Cited by 26 •

2009

A cell death-based assay for PTI in Nicotiana benthamiana plants is described.

Research

JoVE Journal - Biology
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Detection of Histone Modifications in Plant Leaves

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Cited by 19 •

2011

A reliable and useful approach to detect histone modifications on specific plant genes is described. The approach combines chromatin immunoprecipitation (ChIP) and real-time quantitative PCR. It allows detection of histone modifications on specific genes with a role in diverse physiological processes.

Research

JoVE EoE - Bacterial Pathogenesis and Host Interactions

Single-Cell Analysis of Gene Expression from Plant-Extracted Pathogens

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2026

Source: Rufián, J. S., et al. Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022).This video demonstrates a method for analyzing gene expression in plant-extracted pathogenic bacteria at the single-cell level using a fluorescently tagged transcription factor. Confocal imaging reveals heterogeneity in virulence gene expression, highlighting functional diversity within a clonal bacterial population.

Research

JoVE Journal - Bioengineering

Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering

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2023

We engineered, fabricated, and validated an instrument that rapidly processes phloem-rich bark citrus budwood tissues. Compared to current methods, the budwood tissue extractor (BTE) has increased sample throughput and decreased the required labor and equipment costs.

Co-Infiltration Assay for Screening Pathogen Effectors that Suppress Plant RNA Silencing

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2026

Source: Shi, J., et al. Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens. J. Vis. Exp. (2020)This video demonstrates a co-infiltration assay for identifying pathogen effector proteins that suppress RNA silencing in Nicotiana benthamiana 16c. A fluorescent reporter and a candidate effector gene are delivered into plant leaves via Agrobacterium. Normally, RNA silencing degrades reporter transcripts, reducing fluorescence. Suppressor effectors...

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