Adipogenesis
Adipogenesis - Related Videos
08:28
Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
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Cited by 5 •
2017
Here we report a method for isolation of Adipocyte Progenitor Cell (APC) populations from Perivascular Adipose Tissue (PVAT) using Magnetic-activated Cell Sorting (MCS). This method allows for an increased isolation of APC per gram of adipose tissue when compared to Fluorescence-Activated Cell Sorting (FACS).
06:08
Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
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Cited by 2 •
2023
This protocol describes the semi-automated isolation of the stromal vascular fraction (SVF) from murine adipose tissue to obtain preadipocytes and achieve adipocyte differentiation in vitro. Using a tissue dissociator for collagenase digestion reduces experimental variation and increases reproducibility.
05:30
Exogenous Mitochondrial Transfer in Differentiating Brown Adipocytes and AGPAT2-Deficient Preadipocytes
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2026
Exogenous mitochondria of human and murine origin can be internalized by differentiating preadipocytes and tracked throughout adipogenic differentiation using imaging and genetic tools. While these mitochondria physically interact with the endogenous mitochondrial network, they do not increase the expression of markers associated with mature brown adipocytes.
03:03
In Vitro Mouse Bone Marrow Stromal Cell Differentiation into Adipocytes
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2025
This video demonstrates the differentiation of bone marrow stromal cells (BMSCs) into adipocytes. In the two-step process, in the presence of specific growth factors and hormones, undifferentiated BMSCs first differentiate into preadipocytes. The second step involving treatment with glucose and insulin leads to the formation of mature adipocytes with multiple lipid droplets.
08:58
An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples
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Cited by 2 •
2015
Mesenchymal stem cells are usually obtained from bone marrow and require expansion culture. When samples clot before processing, a protocol using the (enzymatic) thrombolytic drug urokinase can be applied to degrade the clot. Thus, cells are released and available for expansion culture. This protocol provides a rapid and inexpensive alternative to resampling.
FAQs
PPARγ and C/EBP proteins act as key transcription factors during adipogenesis, coordinating changes in gene expression that establish adipocyte identity. Their activity helps precursor cells adopt the molecular characteristics of mature fat cells and supports lipid accumulation. Examining these regulators allows genetic studies to connect specific transcriptional programs with the formation and function of adipose tissue.
Differentiation requires more than activation of a single gene. During adipogenesis, coordinated changes in gene expression guide precursor cells through the transition toward mature adipocytes, aligning cell identity with lipid-storage capacity and endocrine signaling. This coordinated regulation is important because it links genetic control to the development of adipose tissue with appropriate metabolic functions.
Genetic regulation influences both the identity of adipocytes and the properties they acquire after differentiation. As transcriptional programs promote lipid accumulation and mature adipocyte characteristics, they also help establish adipose tissue as a site of energy storage and endocrine signaling. Studying these links provides a framework for understanding how gene regulation affects broader metabolic function.
Examining altered regulation of adipocyte formation can help researchers explore how genetic and cellular programs relate to obesity, insulin resistance, and diabetes. The process provides a focused system for connecting transcription-factor activity and adipocyte development with metabolic outcomes. This perspective can reveal how changes in fat-cell formation may contribute to impaired energy balance or metabolic function.
Studies of adipogenesis help determine how precursor cells acquire mature adipocyte characteristics, how transcription factors such as PPARγ and C/EBP proteins regulate that transition, and how lipid accumulation develops alongside cell identity. These outcomes connect molecular changes in gene expression with cellular differentiation, making the process useful for investigating adipose tissue development and metabolic regulation.
Understanding the genetic programs that control adipocyte formation may support tissue-engineering research and strategies intended to modulate fat-cell development. The process provides information about how precursor cells establish adipocyte identity and lipid-storage properties. In therapeutic research, this knowledge is relevant to investigating obesity, insulin resistance, diabetes, and other conditions involving adipose tissue or energy balance.