Crispr Cas9
Crispr Cas9 - Related Videos
02:55
Genetically Modifying CAR T Cells Using a CRISPR-Cas9 System
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2025
The video outlines a process for creating genetically modified CAR T cells through the CRISPR-Cas9 System. Infecting T cells with CRISPR and CAR lentiviruses results in modifications to the target gene and the synthesis of a chimeric antigen receptor or CAR, ultimately leading to the formation of genetically modified CAR T cells.
04:22
A Technique for Gene Editing in Natural Killer Cells Using CRISPR Cas9
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2025
This video demonstrates a technique for Cas9 ribonucleoprotein-mediated genetic modification of primary natural killer (NK) cells. A Cas9 ribonucleoprotein, consisting of a Cas9 endonuclease bound to a guide RNA (gRNA) formed by base pairing a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA), is introduced into primary natural killer cells via electroporation. The ribonucleoprotein targets and cleaves the host DNA at the target site, leading to gene knockout via modification of the...
06:47
Genome Editing in the Yellow Fever Mosquito Aedes aegypti using CRISPR-Cas9
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Cited by 2 •
2025
Here, we describe a detailed protocol for genome editing through embryonic microinjection in the mosquito A. aegypti using the CRISPR-Cas9 technology.
10:07
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
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Cited by 3 •
2022
This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.
05:12
All-in-One CRISPR Genome Editing: A Method for Homology Directed Repair-Based Gene Knock-In in Cultured Cells Using CRISPR-Cas9 System
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2025
In this video, we demonstrate all-in-one CRISPR-Cas9 based genome editing in cultured cells where Cas9 and sgRNA are provided as a single plasmid construct to the cells. The CRISPR-Cas9 system and desired gene to be inserted was introduced in cells through electroporation technique to facilitate successful gene editing.
FAQs
Targeting depends on two linked recognition requirements: the guide RNA must be complementary to the intended DNA sequence, and that sequence must lie next to a protospacer adjacent motif, or PAM. The guide provides sequence-based direction, while the PAM marks an acceptable neighboring site for Cas9 activity. Together, these features determine where the nuclease can create a double-strand break.
The cell’s repair response largely determines the genetic result after Cas9 cleavage. Non-homologous end joining repairs the break in a way that can disrupt the targeted gene, supporting knockout experiments. Homology-directed repair instead uses a specified sequence to introduce a planned change, making it relevant when researchers seek sequence correction rather than simple loss of gene function.
CRISPR-Cas9 can support either loss-of-function or sequence-specific editing because the same cleavage event can be resolved through different repair outcomes. If repair disrupts the target, researchers can infer the gene’s role from reduced or lost function. If homology-directed repair introduces a specified sequence, the experiment can examine the effect of a defined genetic correction.
In genetics, CRISPR-Cas9 links a targeted DNA change to biological interpretation. Gene knockouts can help researchers investigate gene function, while functional screens extend this analysis across many targets to identify patterns of genetic effects. The approach also supports disease modeling, allowing experimental systems to examine how particular genetic changes relate to disease-associated biology.
A basic workflow begins by selecting a DNA region with a complementary guide RNA target and an adjacent PAM. Cas9 is then directed to that site, where it makes a double-strand break. The cell repairs the break through non-homologous end joining or homology-directed repair. Researchers can then interpret the resulting gene disruption or specified sequence change in the context of the experiment.
Disease modeling uses targeted genetic changes to create experimental systems that reflect a disease-relevant alteration. By introducing a disruption or a specified sequence change, researchers can examine the consequences of that alteration and connect genotype with gene function. This makes the technology useful for investigating genetic disease mechanisms and for evaluating potential therapeutic strategies.
In therapeutic research, CRISPR-Cas9 enables investigators to examine specified sequence corrections in disease-relevant genetic studies. Those experiments can be combined with disease modeling and gene-function analysis to evaluate potential therapeutic strategies. Its role is to provide a way to test how targeted genetic changes relate to disease biology and whether a correction merits further investigation.