Probe Alignment
Probe Alignment - Related Videos
13:56
Probe Type II Band Alignment in One-Dimensional Van Der Waals Heterostructures Using First-Principles Calculations
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Cited by 4 •
2019
Calculations performed by the Vienna Ab initio Simulation Package can be used to identify the intrinsic electronic properties of nanoscale materials and predict the potential water-splitting photocatalysts.
09:45
Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies
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Cited by 6 •
2018
Here we present a protocol for wide-area scanning probe nanolithography enabled by the iterative alignment of probe arrays, as well as the utilization of lithographic patterns for cell-surface interaction studies.
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
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Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
07:12
Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment
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Cited by 3 •
2022
This protocol demonstrates the use of a microfluidic channel with changing geometry along the fluid flow direction to generate extensional strain (stretching) to align fibers in a 3D collagen hydrogel (<250 µm in thickness). The resulting alignment extends across several millimeters and is influenced by the extensional strain rate.
08:07
The Microfluidic Probe: Operation and Use for Localized Surface Processing
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Cited by 5 •
2009
In this video we present the microfluidic probe1 (MFP). We explain in detail how to assemble the MFP, mount it atop an inverted microscope, and align it relative to the substrate surface, and finally show how to use it to process a substrate surface immersed in a buffer solution.
FAQs
Lateral error can place the probe beside the intended measurement site, while vertical error changes the distance or contact geometry between the probe and sample. Orientation error can also alter how a probe tip, electrode, optical fiber, or microfluidic interface interacts with the target. Limiting these errors helps maintain localized measurements and reduces variation between repeated experiments.
The positioning stage provides controlled movement, allowing the probe to approach a defined location and geometry. Visual or sensor-based feedback supplies information about the probe and sample relationship during positioning. Together, these components help operators adjust the probe while limiting lateral and vertical displacement, which supports more consistent measurements and manipulations.
Orientation determines how the probe interacts with the sample and whether the intended part of the probe engages the target. This matters for force measurements, localized stimulation, molecular sensing, and characterization of cells or tissues. Maintaining the intended orientation can reduce sample disturbance and probe damage while improving consistency across measurements.
The required geometry depends on the probe component and the task. A probe tip may need controlled positioning for force measurements, whereas an electrode may require placement for localized stimulation or sensing. Optical fibers and microfluidic interfaces likewise must be brought into a defined relationship with the sample or instrument. Alignment therefore adapts to the interface being used.
A typical workflow uses a positioning stage to move the probe toward the intended site while visual or sensor-based feedback guides the adjustment. The operator brings the probe tip or interface into the required geometry, limiting lateral and vertical error during positioning. This controlled approach helps reduce unintended sample disturbance and supports reproducible measurement or manipulation.
Accurate alignment supports several bioengineering tasks, including reproducible force measurements, localized stimulation, molecular sensing, and cell or tissue characterization. It is useful whenever a probe must act at a defined location or interact with a sample in a controlled geometry. The resulting reduction in positional variation improves experimental reliability and comparability between measurements.