Stm Sts Studies
Stm Sts Studies - Related Videos
07:29
Separating Protein with SDS-PAGE
0 Views •
2023
Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis, or SDS-PAGE, is a widely-used technique for separating mixtures of proteins based on their size and nothing else. SDS, an anionic detergent, is used to produce an even charge across the length of proteins that have been linearized. By first loading them into a gel made of polyacrylamide and then applying an electric field to the gel, SDS-coated proteins are then separated. The electric field acts as the driving force, drawing the SDS...
01:27
SDS-PAGE
0 Views •
2023
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field. A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
10:02
Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection
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2025
This protocol standardizes SDS-PAGE analysis for personalized protein corona profiling on nanoparticles, enabling reproducible, scalable, and low-cost detection of cancer-specific signatures. Designed for early diagnosis of pancreatic ductal adenocarcinoma, it offers a practical, REASSURED-aligned alternative to complex proteomic methods in both research and clinical settings.
02:44
Non-Reducing SDS PAGE: A Method to Analyze Disulfide-Linked Multimeric Protein Complexes
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2025
This video describes the technique of separating the protein samples by non-reducing SDS-PAGE to analyze the multimeric protein complexes. This technique retains the multimer subunits of a protein held by the disulfide bonds which can later be analyzed by western blotting.
10:48
SDS-PAGE/Immunoblot Detection of Aβ Multimers in Human Cortical Tissue Homogenates using Antigen-Epitope Retrieval
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Cited by 26 •
2010
We describe a technique for the preparation of clarified human cortical homogenates, protein separation by SDS-PAGE, antigen retrieval and immunoblotting with an antibody to the Aβ peptide. Using this protocol, we consistently detect monomeric and multimeric Aβ in cortical tissue from humans with Alzheimer's pathology.
FAQs
STM measures the bias-driven tunneling current while the conductive tip scans across the surface, providing spatial information about atomic structure and surface features. STS changes the applied voltage and records how the current responds at a selected location. Comparing these measurements connects the observed structure with local electronic behavior, including energy gaps and surface states.
The applied bias drives electron tunneling between the sharp tip and the sample, making the measured current sensitive to the local surface. In STS, varying the bias produces a current-versus-voltage response that helps estimate the local electronic density of states. This voltage-dependent information distinguishes electronic features that may not be apparent from spatial measurements alone.
The spatial measurement can reveal atomic structure, defects, and adsorbates, whereas the spectroscopic response provides information about local electronic states. Energy gaps and surface states therefore become accessible alongside physical features. This distinction matters when two regions appear structurally similar but exhibit different electronic behavior, or when a defect alters local properties without changing the broader surface pattern.
Defects and adsorbates are not merely imaging irregularities; they can be correlated with changes in the local electronic response. Surface states likewise provide electronic features associated with the surface region. Examining spatial and spectroscopic signals together helps researchers separate structural variations from electronic effects, improving interpretation of nanoscale materials and engineered surfaces.
A measurement begins by positioning a sharp, conductive tip close to a conductive sample and applying a bias to generate tunneling current. STM records the current while the tip scans the surface. For STS, the voltage is varied and the resulting current changes are recorded at selected locations. The combined data are then used to examine structure and local electronic behavior.
Engineering researchers can apply these studies to surface analysis, device development, catalyst evaluation, and failure investigation. The measurements identify atomic-scale structure, defects, adsorbates, energy gaps, and surface states in conductors, semiconductors, and nanostructures. These observations help relate nanoscale material behavior to macroscopic performance and can guide interpretation of device or surface problems.