Dip-and-wipe Method

The dip-and-wipe method is a simple surface-treatment technique in which a material is immersed in a liquid formulation and then wiped to remove excess, enabling controlled deposition. Its operation depends on contact between the substrate and solution, followed by wiping that regulates the retained film before drying or curing. In bioengineering, this approach can support the preparation of coated biomaterials, laboratory devices, and interfaces for subsequent biological testing. Because it requires relatively simple equipment and can be adapted to different substrates and formulations, the method offers a practical way to modify surface properties while maintaining straightforward process control and reproducibility.

Dip-and-wipe Method - Related Videos

Research

JoVE Journal - Engineering
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Standardized Method for Measuring Collection Efficiency from Wipe-sampling of Trace Explosives

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Cited by 7 •

2017

Optimized sampling protocols and the development of new wipe materials can be facilitated by standardized measurements of collection efficiency from wipe-sampling. Our approach for sampling trace explosives uses an automated device to control speed, force, and distance during wipe-sampling followed by extraction of collected explosives.

Research

JoVE Journal - Immunology and Infection

Nasal Wipes for Influenza A Virus Detection and Isolation from Swine

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Cited by 19 •

2015

The authors present a protocol to collect swine nasal wipes to detect and isolate influenza A viruses.

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression

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Cited by 20 •

2010

This article illustrates the floral-dip method of Agrobacterium tumefaciens -mediated transformation of Arabidopsis thaliana. By introducing a cell-cycle regulated promoter-reporter, pTSO2::β-glucuronidase (GUS), into Arabidopsis, we illustrates how one detects GUS reporter expression in transgenic seedlings.

Research

JoVE Journal - Biology
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Floral-Dip Transformation of Flax (Linum usitatissimum) to Generate Transgenic Progenies with a High Transformation Rate

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Cited by 38 •

2014

Here, we present a protocol to transform flax using Agrobacterium-mediated plant transformation via floral-dip. This protocol is simple to perform and inexpensive, yet yields a higher transformation rate than the current available methods for flax transformation.

Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula

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Cited by 10 •

2019

Agrobacterium-mediated transformation using a floral-dip method can be successfully employed to create stable transgenic lines of the extremophyte model Schrenkiella parvula. We present a protocol modified from that for Arabidopsis thaliana, considering different growth habits and physiological characteristics of the extremophyte.

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