Knife-edge Alignment

Knife-edge alignment is an optical technique for locating and centering a laser beam or determining its focal position with high precision. A sharp edge is translated across the beam while a detector records the transmitted power; the resulting intensity transition identifies the beam center, and measurements taken through focus can characterize beam size and waist location. In engineering, this method supports alignment of lenses, mirrors, apertures, and optical systems, particularly when direct imaging is difficult. Its simple hardware, quantitative output, and compatibility with automated stages make it useful for laser characterization, precision positioning, and optical system calibration.

Knife-edge Alignment - Related Videos

Research

JoVE Journal - Bioengineering
Free Sample

Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

0 Views •

Cited by 16 •

2011

The full process from brain specimen preparation to serial sectioning imaging using the Knife-Edge Scanning Microscope, to data visualization and analysis is described. This technique is currently used to acquire mouse brain data, but it is applicable to other organs, other species.

Research

JoVE Journal - Medicine

Clinical Efficacy of Small Needle Knife Therapy on Stage I-II Frozen Shoulder

0 Views •

Cited by 6 •

2023

The protocol presented here describes the detailed operation method and proves the effectiveness of a small needle knife in the treatment of frozen shoulders.

Research

JoVE Journal - Medicine

Use of the Scissor-Type Knife During the Peroral Endoscopy Myotomy Procedure for the Treatment of Achalasia

0 Views •

2023

To minimize the technical difficulty and improve the safety of peroral endoscopic myotomy (POEM), we describe a protocol for using a scissor-type knife for the main steps of POEM, including mucosal incision, submucosal tunneling, myotomy, and hemostasis.

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

0 Views •

Cited by 3 •

2022

This protocol demonstrates the use of a microfluidic channel with changing geometry along the fluid flow direction to generate extensional strain (stretching) to align fibers in a 3D collagen hydrogel (<250 µm in thickness). The resulting alignment extends across several millimeters and is influenced by the extensional strain rate.

Research

JoVE Journal - Biology
Free Sample

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

0 Views •

Cited by 21 •

2009

The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.

View All Results

FAQs

Related Topics