Plug Transfer Technique
Plug Transfer Technique - Related Videos
01:22
Formation of the Platelet Plug
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2024
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss. As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
11:43
Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
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Cited by 24 •
2012
When working in a laboratory, it is imperative to minimize sources of contamination. Aseptic technique refers to procedures that permit transfer of cultures and reagents while avoiding contact with non-sterile surfaces. Serological pipettes and micropipettors are used to measure precise volumes without compromising sterility of solutions used in experiments.
09:03
Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies
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Cited by 1 •
2023
The method presented here can evaluate the effect of reagents on angiogenesis or vascular permeability in vivo without staining. The method uses dextran-FITC injection via the tail vein to visualize neo-vessels or vascular leakage.
08:16
In Vivo Study of Human Endothelial-Pericyte Interaction Using the Matrix Gel Plug Assay in Mouse
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Cited by 11 •
2016
We present a protocol to study human endothelial-pericyte interactions in mouse using a variation of the matrix gel plug angiogenesis assay.
07:03
Bilayer Microfluidic Device for Combinatorial Plug Production
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2023
The fabrication of a polydimethylsiloxane (PDMS)-based bilayer device for the production of combinatorial libraries in water-in-oil emulsions (plugs) is presented here. The necessary hardware and software required to automate plug production are detailed in the protocol, and the production of a quantitative library of fluorescent plugs is also demonstrated.
FAQs
Consistent plug dimensions help ensure that each transfer begins with a comparable amount and arrangement of material. This reduces variation caused by differences in sample size or shape, making comparisons between treatments or locations more reproducible. In environmental experiments, standardized plugs support clearer evaluation of biological activity, growth, colonization, and responses to changing conditions.
Preserving the plug’s structure and composition helps maintain the physical and biological features present at the original location. Excessive compression or disturbance could alter how the transferred material interacts with its new medium or substrate. Limiting these changes makes subsequent observations more representative of the material’s response rather than effects introduced during handling.
The tool should be appropriately sized for the material being moved and the receiving cavity or substrate. A suitable match helps produce a defined plug that can be aligned and placed without unnecessary compression or disruption. This supports consistent positioning across samples, which is important when environmental treatments or locations are being compared.
A typical workflow uses a sterile, appropriately sized tool to remove the selected plug, align it with a receiving cavity or substrate, and place it with minimal compression. The sequence is designed to preserve the plug during movement and create consistent placement. Careful handling supports reproducibility when transferred material is evaluated under controlled conditions.
Environmental researchers can use this technique when they need to move soil, sediment, plant, or microbial material while retaining a defined sample structure. It supports cultivation, comparison, and experimental manipulation across media or locations. The controlled format is especially useful when researchers want to examine how transferred material behaves under different environmental conditions or treatments.
After transfer, researchers can evaluate biological activity, growth, colonization, or broader environmental responses. Because plug size and placement can be standardized, observed differences are easier to relate to the experimental conditions rather than inconsistent handling. The approach therefore supports comparisons among materials, receiving substrates, or manipulated environments while retaining the transferred sample’s defined form.