Spatial Smoothing

Spatial smoothing is a statistical and computational technique that reduces local noise in spatially organized data while preserving broader patterns. It works by replacing each observation with a weighted average of nearby observations, often using a moving window or kernel whose bandwidth determines how strongly neighboring locations influence the result. In behavioral research, spatial smoothing can clarify patterns in animal movement, habitat use, social interactions, or activity distributions by converting variable point measurements into interpretable spatial maps. Appropriate smoothing improves visualization and pattern detection, but excessive smoothing can obscure meaningful behavioral boundaries or small-scale changes.

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Research

JoVE Journal - Biochemistry
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Spatial Molecular Imaging of the Glycome Using Mass Spectrometry

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2025

This protocol details the key steps to enable rigorous and reproducible measurement of both glycogen and N-linked glycans using mass spectrometry imaging.

Education

JoVE Science Education - Psychology

Spatial Cueing

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2023

Source: Laboratory of Jonathan Flombaum—Johns Hopkins University Attention refers to the limited human ability to select some information for processing at the expense of other stimuli in the environment. Attention operates in all sensory modalities: vision, hearing, touch, even taste and smell. It is most often studied in the visual domain though. A common way to study visual attention is with a spatial cueing paradigm. This paradigm allows researchers to measure the consequences of focusing...

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis

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2025

Here, we present a method for aligning and cryosectioning multiple Zebrafish (Danio rerio) larvae samples and collecting them on a single slide for spatial transcriptomic analysis.

Spatial Memory Testing Using Mazes

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2023

Spatial learning and memory are neurological functions that allow us to remember important details associated with our environment. Scientists test this phenomenon in rodents using different types of mazes like Morris water maze, Radial water maze, and Barnes maze. By investigating spatial memory in rodents, neurobehavioral scientists can gain valuable understanding of how these processes are altered following brain damage in humans. Here, the neurobiology behind spatial memory is briefly...

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

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2025

This article introduces a protocol for using DeepSpaceDB, a dynamic, interactive database for spatial transcriptomics, offering analysis workflows and examples to explore tissue organization and disease-related gene expression.

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