3d Epithelial Tissues
3d Epithelial Tissues - Related Videos
11:12
Tissue Engineering of a Human 3D in vitro Tumor Test System
0 Views •
Cited by 34 •
2013
Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.
12:22
Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models
0 Views •
Cited by 81 •
2012
A rotating cell culture system that allows epithelial cells to grow under physiological conditions resulting in 3-D cellular aggregate formation is described. The aggregates generated display in vivo-like characteristics not observed in conventional culture models and serve as a more accurate organotypic model system for a multitude of scientific investigations.
09:46
Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip
0 Views •
Cited by 2 •
2023
The present protocol describes the capabilities and the essential culture modalities of the Open-Top Organ-Chip for the successful establishment and maturation of full-thickness organ-on-chip cultures of primary tissues (skin, alveolus, airway, and intestine), providing the opportunity to investigate different functional aspects of the human epithelial/mesenchymal and vascular niche interface in vitro.
08:49
Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
0 Views •
Cited by 6 •
2016
This manuscript describes a soft lithography-based technique to engineer uniform arrays of three-dimensional (3D) epithelial tissues of defined geometry surrounded by extracellular matrix. This method is amenable to a wide variety of cell types and experimental contexts and allows for high-throughput screening of identical replicates.
09:18
Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion
0 Views •
2025
There is a critical need for 3D cancer models that capture heterocellular crosstalk to study cancer metastasis. Our study presents the generation of heteromulticellular stromal-epithelial in a scaffold and scaffold-free environment that can be used to study invasion and cellular spatial distributions.
FAQs
Apical-basal polarity gives epithelial cells an organized orientation, while cell-cell junctions connect neighboring cells into a coordinated layer. Together, these features help the tissue establish barrier behavior and regulate how substances move across or between cells. Their preservation is therefore important when using engineered models to study epithelial transport, secretion, or tissue responses.
The extracellular matrix provides a surrounding environment with which epithelial cells interact as they organize in three dimensions. These interactions contribute to the formation of spheroids, organoids, or layered tissues rather than isolated cellular arrangements. In bioengineering, incorporating matrix interactions helps models represent tissue organization and remodeling more realistically than a flat culture alone.
Three-dimensional organization allows epithelial cells to establish spatial relationships, barrier structures, and interactions with their surrounding matrix. As a result, models can represent transport, secretion, and tissue remodeling in a more tissue-like setting. This added organization is especially relevant when chemical or mechanical stimuli produce responses that depend on the structure of the epithelial layer.
Chemical and mechanical stimuli can be examined through the changes they produce in epithelial behavior and tissue organization. Because 3D models preserve polarity, junctions, and matrix interactions, they provide a setting for studying responses within an organized tissue rather than within dispersed or planar cells. This supports investigations of how epithelial structures adapt or remodel under experimental conditions.
Bioengineers should consider whether the model reproduces apical-basal polarity, cell-cell junctions, extracellular-matrix interactions, and relevant barrier functions. They should also match the structure, such as a spheroid, organoid, or layered tissue, to the question being studied. These characteristics determine whether the model can meaningfully represent transport, secretion, remodeling, or stimulus responses.
They are useful when toxicity needs to be evaluated in a tissue-like epithelial context rather than only in a two-dimensional cell layer. Their three-dimensional organization can support assessment of effects on barrier functions, transport, secretion, or remodeling. This makes them relevant platforms for investigating how chemical exposures influence epithelial tissues during biomedical and bioengineering research.
These models can guide the design of tissue-engineered grafts by showing how epithelial cells organize, establish junctions, interact with extracellular matrix, and maintain barrier-related functions. Their capacity to form layered tissues or other organized structures also supports regenerative-medicine research. Such information can help evaluate whether engineered constructs reproduce important features of epithelial tissue organization.