Eye Biodistribution
Eye Biodistribution - Related Videos
13:29
Biodistribution of Nano-drug Carriers: Applications of SEM
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2023
Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut Nanoparticles have been increasingly used research towards targeted drug delivery and controlled drug release. While most of these particles have been developed as polymeric or liposomal particles because of their biocompatibility, there is a trend in current research toward the use of metallic and magnetic nanoparticles. These metallic nanoparticles...
06:15
An In Vivo Immunofluorescence Localization Method to Study Antibody Biodistribution
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2025
This video demonstrates an in vivo immunofluorescence localization (IVIL) technique to study the biodistribution of tumor-targeting antibodies in a murine model of breast cancer. Upon the intravenous injection of a tumor-specific antibody in the mouse, the tumor is surgically isolated, and the localization of the antibody within the tumor tissue is confirmed via ex vivo immunostaining.
13:35
Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules
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Cited by 26 •
2017
This work describes methods to establish acute and chronic hyperglycemia models in zebrafish. The aim is to investigate the impact of hyperglycemia on physiological processes, such as constitutive and injury-induced neurogenesis. The work also highlights the use of zebrafish to follow radiolabeled molecules (here, [18F]-FDG) using PET/CT.
04:06
A Rat Dry Eye Model with Lacrimal Gland Dysfunction Induced by Scopolamine
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Cited by 3 •
2024
Here, we establish a rat model of lacrimal gland dysfunction to provide a basis for the study of aqueous-deficient dry eye.
05:59
Obtaining High-Quality Cryosections of Whole Rabbit Eye
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Cited by 3 •
2023
This protocol describes a reliable method for obtaining high-quality cryosections of whole rabbit eyes. It details rabbit eye dissection, fixation, embedding, and sectioning procedures, which may be easily adapted for use in any study utilizing immunohistochemistry in larger eyes.
FAQs
The administration route affects which ocular tissues a material encounters first and how easily it can reach deeper regions. Route-dependent exposure interacts with tissue barriers, fluid movement, and clearance pathways, so the same drug or biologic may show different patterns of accumulation and loss depending on how it is delivered. This comparison helps bioengineers select delivery strategies suited to a target tissue.
The cornea and blood-retinal barrier restrict movement between compartments of the eye and surrounding circulation. Their barrier properties can limit tissue exposure even when a material is present near the eye. Evaluating these interfaces helps explain why some formulations remain localized while others reach posterior tissues, informing designs intended to improve access without increasing unwanted distribution.
A material’s physicochemical properties influence how it diffuses, interacts with cells, and follows ocular fluid flows. These factors can alter where the material accumulates and how readily cells take it up. Bioengineers therefore consider material properties alongside delivery route and tissue barriers when designing nanoparticles, biologics, implants, or injectable formulations for controlled ocular exposure.
Ocular distribution reflects a balance between transport and removal. Diffusion and fluid flow move materials through tissue spaces, while cellular uptake can change their local retention. Tears and circulation provide important clearance pathways that reduce exposure. Understanding which processes dominate in a given design helps researchers interpret accumulation patterns and identify reasons a treatment may have limited persistence.
Analysis can show which ocular tissues receive a material, where it accumulates, and how exposure changes as clearance occurs. These observations connect a delivery design with its tissue-level distribution rather than relying only on the administered dose. The resulting information supports evaluation of therapeutic exposure, potential off-target distribution, and whether a formulation reaches the intended anatomical site.
In bioengineering, distribution data guide the design and refinement of targeted ocular delivery systems. Researchers can use tissue exposure patterns to assess implants, injectable formulations, gene delivery platforms, nanoparticles, and other treatments. This context is especially relevant when developing therapies for eye diseases because improved localization may support efficacy while limiting exposure to tissues outside the intended target.