Rp 13 Software

RP 13 Software is a specialized computational tool used to support data handling, analysis, or design workflows in bioengineering, where reliable digital processing helps connect experimental measurements with engineering decisions. The software typically converts user-entered parameters or instrument-generated data into organized analyses, visualizations, or calculated outputs through programmed algorithms and defined processing steps. Its use can improve reproducibility, reduce manual errors, and help researchers evaluate biological systems, optimize engineered designs, and communicate results. Understanding the software’s inputs, processing logic, and outputs is important for interpreting analyses accurately and integrating computational methods into bioengineering research.

Rp 13 Software - Related Videos

Research

JoVE EoE - Prostate Cancer

Peptide Purification: An RP-HPLC-based Technique to Extract Peptides from Digested Protein Lysates

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2023

In this video, we demonstrate reversed-phase high-performance liquid chromatography to purify peptides from a digested protein lysate mixture. These purified peptides are then lyophilized and stored for downstream applications. Analysis of peptides can help understand the mechanics involved in cellular signaling and cancer.

Research

JoVE Journal - Biology

Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy

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2023

We describe an experimental setup for administrating hyperpolarized 13C-labeled metabolites in continuous perfusion mode to an isolated perfused mouse heart. A dedicated 13C-NMR acquisition approach enabled the quantification of metabolic enzyme activity in real-time, and a multiparametric 31P-NMR analysis enabled the determination of the tissue ATP content and pH.

Research

JoVE Journal - Bioengineering
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Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR

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Cited by 2 •

2025

This protocol integrates a custom-built bioreactor system with hyperpolarized ¹³C NMR spectroscopy to measure real-time pyruvate metabolism in live cells, improving reproducibility and kinetic accuracy for metabolic studies and drug screening applications.

Education

JoVE Core - Analytical Chemistry

Carbon-13 (¹³C) NMR: Overview

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2024

Carbon-13 is a naturally occurring NMR-active isotope of carbon with a low natural abundance of 1.1%. In contrast, carbon-12 is the most abundant isotope of carbon with zero nuclear spin. Therefore, it is NMR inactive. The gyromagnetic ratio of carbon-13 is smaller than that of protons. As a result, carbon-13 resonance is about 6000 times weaker than proton resonance. For a given magnetic field strength, the resonance frequency of carbon-13 is about one-fourth of the resonance frequency for...

Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test

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Cited by 18 •

2013

Determination of gastric emptying with a non-invasive [13C]-octanoic acid breath test for tracking gastroparesis in female NOD LtJ mice.

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