Extended Depth Software

Extended Depth Software is an image-processing tool that produces a single focused image from samples whose structures extend beyond the microscope’s depth of field. It works by combining images captured at different focal planes, identifying the sharpest regions in each, and merging them into an extended-depth composite. In biology, this approach helps researchers visualize thick or uneven specimens, including organisms, tissues, and microscopic structures, without restricting the image to one focal level. The resulting composites can improve documentation, morphological analysis, and communication of spatial features, supporting microscopy workflows in teaching, research, and biological imaging.

Extended Depth Software - Related Videos

Education

JoVE Core - Civil Engineering

Uniform Depth Channel Flow

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2025

Uniform depth channel flow keeps fluid depth consistent along channels such as irrigation canals. In natural channels, such as rivers, approximate uniform flow is often assumed. This condition occurs when the channel’s bottom slope matches the energy slope, balancing potential energy lost from gravity with head loss due to shear stress. This balance prevents depth changes along the channel length, resulting in a steady, uniform flow.Uniform flow in open channels with a constant cross-section...

Depth Perception and Spatial Vision

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2024

Depth perception is the ability to perceive objects three-dimensionally. It relies on two types of cues: binocular and monocular. Binocular cues depend on the combination of images from both eyes and how the eyes work together. Since the eyes are in slightly different positions, each eye captures a slightly different image. This disparity between images, known as binocular disparity, helps the brain interpret depth. When the brain compares these images, it determines the distance to an object.

Research

JoVE Journal - Biology

Extended 78% Hepatectomy in a Mouse Surgical Model

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2024

The mouse model of partial 2/3 (66%) hepatectomy is well described in the literature, but more extended hepatectomies mimicking small-for-size syndrome after liver transplantation have seldom been used. We describe an extended 78% hepatectomy procedure in a mouse model that results in approximately 50% postoperative lethality in healthy mice.

Research

JoVE Journal - Cancer Research
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Three-Dimensional Imaging of Tumor-Bearing Tissue Using the Iterative Bleaching Extends Multiplexity Approach

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Cited by 1 •

2025

This article describes a protocol for multiplex immunofluorescence optimized to characterize the three-dimensional architecture of peritoneal metastases.

Uniform Depth Channel Flow: Problem Solving

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2025

To calculate the flow rate for a trapezoidal channel, first, identify the bottom width, side slope, and flow depth of the channel. The cross-sectional area (A) corresponding to the depth of flow (y), channel bottom width (B), and side slope (θ) is determined by:Next, calculate the wetted perimeter, which includes the bottom width and the sloped side lengths in contact with the water. Using the values of the cross-sectional area and the wetted perimeter, determine the hydraulic radius by...

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